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KMID : 0545120020120061010
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 6 p.1010 ~ p.1012
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Low-Dose Gamma Irradiation as Means of Isolating Carotenoid-Hyperproducing Yeast Mutant
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SUN, NAMKYU
LEE, SEUNGHEE/AHN, GILHWAN/WON, MISUN/SONG, KYUNG BIN
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Abstract
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In order to isolate carotenoid-hyperproducing yeast, low-dose gamma irradiation was used as means of mutagenesis. Phaffia rhodozyma was treated by gamma irradiation of less than 10 kGy, which is considered to be a wholesome irradiation condition established by the Food and Drug Administration. Through repeated rounds of gamma irradiation and visual screening, mutant 3A4-8 was obtained. It produced a 3,824 ㎍ carotenoid/g yeast 59% higher content than 2,265 ㎍/g yeast of the unirradiated one. This result indicates that low-dose gamma irradiation could be used as means of mutagenesis to obtain carotenoid-hyperproducing strain of Phaffia rhodozymam, since only carotenoid-hyperproducing yeast survived gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.
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KEYWORD
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